Undesired AAV sample characteristics to consider when optimizing the downstream process
ALl these types of contaminants can easily be identified and quantified by using the automated image analysis software tools in MiniTEM
After 1st purification step
After 2nd purification step
After final purification step
Large size impurities
Small size impurities
Aggregates
Residual DNA
MiniTEM automatically images, detects and classifies particles and debris. Since automation enables detection and analysis of a far larger number of particles than is feasible with manual operation, the results are more consistent, statistically significant and reproducible.
Undetected particles
Detected primary particles and debris
Quantified particles
Time saving through automated MIniTEM analysis
The proportion of broken AAV capsids in a sample can be measured using MiniTEM as negative stained samples of AAV particles display variations in staining patterns correlated to capsid integrity status.
Intact AAV particles appear as lacking internal staining as opposed to broken particles that exhibit internal dark staining due to disrupted virus capsid structures.
Contact
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113 30 Stockholm
SWEDEN
E-mail: info@vironova.com