Particle integrity analysis

Biologic materials such as viruses are delicate and all processing steps involve shear stresses to the product. For some virus types such as Adenovirus and AAV, broken particles may still appear circular and have the expected size but TEM provides unmatched information on the true status of the virus particles.  

Gene therapy & virus based vaccines

Virus integrity status
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MiniTEM can be used to automatically quantify disrupted empty AAV particles

Virus particle morphology

TEM analysis of negatively stained AAV samples (nsTEM) is a reproducible method for revealing particles with disrupted capsid structure. Disrupted particles take in stain and can therefore be identified by their darker centres. 

 

Comparing particle integrity of two AAV samples in MiniTEM:

Integrity graphs

Integrity states

 

Comparing particle integrity of Adenovirus samples in MiniTEM:

Integrity ratio

 

Below is an analysis of MuLV (murine leukemia virus). The numbers of circular intact particles with preserved morphology, of non-circular particles morphologically affected by the processing, and of debris, are compared.

MuLV debris

 

This analysis is part of our standard service offering. If you wish to have your own automated solution – take a closer look at our MiniTEM system.

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