Generic selectors
Exact matches only
Search in title
Search in content
Search in posts
Search in pages
Filter by Categories
Events
News
Publications

AAV and adenovirus particle integrity analysis

Biologic materials such as viruses are delicate and all processing steps involve shear stresses to the product. For some virus types such as Adenovirus and AAV, broken particles may still appear circular and have the expected size but TEM provides unmatched information on the true status of the virus particles.  

Gene therapy & virus based vaccines

Virus integrity status
Arrow 33.4 Created with Sketch.

MiniTEM can be used to automatically quantify disrupted empty AAV particles

Virus particle morphology

TEM analysis of negatively stained AAV samples (nsTEM) is a reproducible method for revealing particles with disrupted capsid structure. Disrupted particles take in stain and can therefore be identified by their darker centres. 

 

Comparing particle integrity of two AAV samples in MiniTEM:

Integrity graphs

Integrity states

 

Comparing particle integrity of Adenovirus samples in MiniTEM:

Integrity ratio

 

Below is an analysis of MuLV (murine leukemia virus). The numbers of circular intact particles with preserved morphology, of non-circular particles morphologically affected by the processing, and of debris, are compared.

MuLV debris

 
Bacteriophages
Arrow 33.4 Created with Sketch.

Sample: Myoviridae Coliphage - Isolated by the Brouns lab, Kavli Institute - BN/TU Delft

This analysis is part of our standard service offering. If you wish to have your own automated solution – take a closer look at our MiniTEM system.

Electron microscopy services

Electron microscopy services specialized for biopharmaceuticals

MiniTEM™

Automated transmission electron microscopy analysis

Contact us

Receive expert advice on how TEM can provide insights into your samples.

Sample description (optional)

Analysis objective (optional)

Services

Study Proposal Form

Please fill out the following information with as much detail as you can, in order for us to provide you with an accurate quote as soon as possible.

* - Compulsory fields
What is the nature of the product
If relevant, where will the product be marketed
Should the study be performed in compliance with GLP
Should the study be performed in duplicate
Will a representative from your company be present during the testing
Process steps to be tested Model viruses Processing time Number of points for sampling If representative(s) will be present, please specify for which step(s)
Vironova Biosafety
Thank you for your interest.