Particle size distribution and purity analyses

A size distribution study can be helpful when detection of aggregation is of interest, for instance, due to unsuitable upstream or downstream conditions or to detect the amount of unsuccessfully removed host cell debris in the downstream purification process. Or simply if want to know whether your virus particles have the expected size.

Liposome & LNP based drugs

Particle size distribution
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Size distribution of liposomes detected with VAS

Gene therapy & virus based vaccines

Size distribution analysis
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MiniTEM automates imaging, AAV particle detection and measurements to present accurate size distribution data

MiniTEM™ can reveal contaminants in AAV samples, for example proteosomes in the figure below.

Graphs

In the above graph an overlay of the results from MiniTEM and DLS analysis of the same sample is shown. DLS was in this case not able to distinguish between AAV particles and the proteasomes.

Size distribution study with MiniTEM can also reveal small size debris and aggregates

Level and size of aggregates

The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to what is revealed in the acquired images.

 

Biologics

Particle size distribution of recombinant proteins
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MiniTEM is employed for size distribution analysis of recombinant proteins to detect deviations in size

The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to the content of the acquired images.

 
Aggregate & particle detection
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TEM imaging of different samples of monoclonal antibodies (mAbs)

Single mAbs in a monolayer can be observed as well as stress induced aggregates.

Material sciences

Size distribution
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Automated MiniTEM size analysis of nanoparticles in material science is 5-10 times faster than manual TEM.

Kaolin

Gold nanoparticles

Calcium carbonate

 
 
Particle size A
Particle size B

The analyses above are examples of those that we offer as a service. If you wish to have your own in-house automated solution – take a closer look at our MiniTEM system.