Particle size distribution and purity analyses
A size distribution study can be helpful when detection of aggregation is of interest, for instance, due to unsuitable upstream or downstream conditions or to detect the amount of unsuccessfully removed host cell debris in the downstream purification process. Or simply if want to know whether your virus particles have the expected size.
Liposome & LNP based drugs
Size distribution of liposomes detected with VAS
Gene therapy & virus based vaccines
MiniTEM automates imaging, AAV particle detection and measurements to present accurate size distribution data
MiniTEM™ can reveal contaminants in AAV samples, for example proteosomes in the figure below.
In the above graph an overlay of the results from MiniTEM and DLS analysis of the same sample is shown. DLS was in this case not able to distinguish between AAV particles and the proteasomes.
Size distribution study with MiniTEM can also reveal small size debris and aggregates
The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to what is revealed in the acquired images.
Biologics
MiniTEM is employed for size distribution analysis of recombinant proteins to detect deviations in size
The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to the content of the acquired images.
TEM imaging of different samples of monoclonal antibodies (mAbs)
Single mAbs in a monolayer can be observed as well as stress induced aggregates.
Material sciences
Automated MiniTEM size analysis of nanoparticles in material science is 5-10 times faster than manual TEM.
Kaolin |
Gold nanoparticles |
Calcium carbonate |
