Lentiviral vectors

Harness the power of Transmission Electron Microscopy (TEM) to unlock comprehensive insights into your lentiviral vectors. Whether you're examining overall morphology, particle integrity, size distribution, packaging analysis, or even aggregation of your products, our services offer unparalleled precision and reliability.

Discover the power of precision with our CryoTEM services, an innovative solution for classifying lentiviral vectors

Experience the captivating world of lentiviral vector particles meticulously captured to showcase a diverse range of particles. Explore those with spiked surfaces and high internal density, as well as intriguing low-density variants. Uncover liposome-like particles with versatile internal densities. The subtle granular background adds an artistic touch, while our AI-driven quality control ensures exceptional image accuracy. Discover the unique beauty and limitless potential of each particle, igniting your imagination on an incredible journey of endless possibilities.

Our CryoTEM technique harnesses the power of rapid particle fixation within vitreous ice, followed by imaging under cryogenic conditions. This GMP-compliant process preserves the particle structure in an almost native state, providing an accurate reflection of the vector's structure. With our AI-based unbiased classification, we're able to distinguish various particle features, including the internal structure of the vectors. This valuable information enables us to classify your particles into distinct categories: High-density particles, Low-density particles, Lentiviral particles exhibiting a visible core, Liposome-like particles, and others depending on the phase of production.

Our CryoTEM images offer a vivid representation of lentiviral particles, showcasing the original images and their corresponding classified images. Through our unique color-coding system, you can easily identify the different particle types:

• HDP (High-density lentivirus particle): Particles having traceable spikes and displaying a higher internal density than the image background (Red).
• LDP (Low-density lentivirus particle): Particles having traceable spikes, and displaying a distinct outer shell and less dense interior similar to the image background (Blue).
• LP (Liposome-like particle): Particles having no trace of spikes and either high or low internal density (Yellow).
• UNT (Uncertain): Particles that could not be unambiguously classified as either of the above types (Black).

Unveiling the Dimensions: A Deep Dive into Lentiviral Particle Size Distribution

As a GMP-certified service, we offer Particle Size Distribution Analysis, a service designed to reveal the spectrum of particle sizes within your sample, facilitating accurate classification into distinct categories. From nanoscale lentiviral vectors to larger entities, our measurements deliver precise information about your sample's size variability, leading to better decision-making and optimized product outcomes.

Our state-of-the-art semi-automatic detection and size analysis system revolutionizes particle analysis within the sample and generate size distribution plots (right) and statistics. The original image (left) is compared to the detected image((left), with identified particles highlighted by red circles.  With our advanced solution, you can effortlessly analyze particle sizes, identify trends, and make data-driven decisions.

The Power of Precision: GMP-Certified nsTEM Analyses and AI-Driven Insights for Enhanced Quality Control

Our GMP-certified nsTEM analyses are an AI-based approach that delivers comprehensive information that directly correlates with the safety and efficacy of your lentiviral vector product. Our cutting-edge VAS software integrates seamlessly with our nsTEM analyses, facilitating precise quantification of cluster, aggregate, and individual particle ratios. This potent combination not only illuminates the finer details of your vectors but also significantly enhances your quality control measures.

AI-Enhanced nsTEM Analysis: Unlocking Quality Control Potential for Aggregation Analysis

With our AI-enhanced approach, we not only provide high-quality images but also deliver crucial data that directly correlate with your product's safety and efficacy. Whether you're examining clusters, aggregates, or individual particles, our nsTEM analyses serve as a reliable foundation for your quality control measures. This amalgamation of quantitative and qualitative data equips you with the knowledge to guide processing decisions about purification and storage with more confidence.

Our representative images and histograms visually illustrate the characteristic morphological structures of lentiviral particles, and the distribution of clusters /aggregates (red), and individual particles (green). The detected and classified particles are overlaid with color-coded outlines, offering a clearer understanding of your sample.

FAQ

Can TEM distinguish a lentiviral vector from other membraneous structures?
The outer membrane of lentiviral vectors is characterized by the presence of outwardly protruding spikes, which are not typically found on other membranous structures associated with lentiviral vectors. These spikes can be observed and visualized using both negative stain TEM (nsTEM) and cryo-electron microscopy (cryoTEM).

Can the internal structure of lentiviral vectors be observed with TEM?
The internal structures, such as the capsid, of lentiviral vectors can be effectively observed using cryo-TEM, but not with nsTEM.

From what stage of production do you recommend sending the lentiviral vector sample to Vironova for analysis?
After the initial purification stage, all samples can be subjected to analysis using transmission electron microscopy (TEM).

Will the stain use in nsTEM damage the lentiviral particles?
We have developed a well-established protocol that minimizes stain-induced artifacts and ensures the integrity of the lentiviral particles during imaging.

Need Quality Control with TEM Analysis