Liposomes and drug delivery systems

Ensure the safety and efficacy of your liposomes by leveraging the power of our advanced cryo-TEM analyses. Our cutting-edge analytical technology accurately assesses the crucial quality parameters including the overall morphology, lamellarity, membrane thickness, packaging, and circularity. 

Accurate Assessment of Liposome Packaging with TEM

Our cryo-TEM analyses offer a detailed view of the packaging of your liposomes, providing valuable insights into their encapsulation efficiency. This data is crucial for evaluating the performance of drug delivery systems and is delivered conveniently to you in a comprehensive analytical report.

Uncover the Hidden Intricacies: See for yourself the stark difference in morphology between an empty liposome and a liposome containing a drug crystal inside. Our detailed cryo-TEM images bring you right into the heart of the liposome, revealing the complexities that are crucial for your research.

Our semi-automated detection system presents a representative histogram (left) that effectively displays the liposome packaging. The corresponding image (right) simplifies data interpretation by overlaying detected and classified particles with clear blue/green/red outlines. This allows for a visual, user-friendly understanding of encapsulation efficiency. The percentage of encapsulation can be correlated to the efficiency of the drug release. This data is provided in the analytical report.

Lamellarity Assessment with Precision

Understanding the lamellarity of your liposomes is essential for predicting drug release kinetics. Our cryo-TEM technology, combined with the sophisticated analysis of our AI-powered VAS software, distinguishes liposomes based on their lamellarity with pinpoint accuracy. 

Our cryo-TEM images display the morphology of liposomes with different lamellarities, illustrating the clear distinction between unilamellar, multilamellar, and multiparticle liposomes. These high-resolution images provide an enhanced understanding of the structural characteristics that impact drug release kinetics.

A representative histogram (left) efficiently displays the various lamellarities. The corresponding image (right) brings the data to life by overlaying detected and classified particles with vibrant red/yellow/green outlines, facilitating an easy interpretation of liposome structure. Whether your liposomes are unilamellar, multilamellar, or multiparticle, we provide you with precise and reliable data.

Maintain Quality Control with Reliable Size and Internal Volume Measurements

In liposome research, size and internal volume are key. Thanks to our AI-powered VAS software used in conjunction with cryo-TEM, you can ensure the highest level of quality control by obtaining reliable measurements of these crucial attributes. Our software provides clear, easy-to-understand visualizations of these measurements, enabling you to maintain top-notch quality control with ease.

Dive into detailed insights with our representative histograms showcasing the size distribution (left) and internal volume (right) of liposomes. These vital metrics, derived from the semi-automated detection of approximately 1,500 particles, provide comprehensive data necessary for your liposome formulation and research optimization. Our technology brings precision and reliability to your fingertips.

Ensuring Circularity Distribution for Optimal Function

The circularity of liposomes impacts their behavior in biological systems and their drug delivery efficiency. Our advanced cryoTEM analyses allow you to assess the circularity of your liposomes, providing you with critical data to ensure the safety and efficacy of your formulation.

Experience the precision of our VAS software through a representative histogram (left) that displays the circularity of Doxorubicin-containing liposomes. This is complemented by a cryo-TEM image (right), the basis of our analysis, illustrating the intricate details of liposome structure. Our advanced tools provide you with actionable data to guide your research and optimize your liposome formulation.

Maximize Efficiency and Efficacy with Precise Measurement of Lipid Bilayer Thickness

Recognizing the significant impact of lipid bilayer thickness on the efficiency and efficacy of liposomal products, we've made it an essential parameter to monitor in the design of liposome-based drug carriers. By harnessing the capabilities of both cryoTEM and our proprietary VAS software, you can measure the thickness of the lipid bilayer with unmatched precision.

Our proprietary VAS software accurately measures the liposomal bilayer thickness distribution, as shown in our representative histogram. To give you a comprehensive view of liposomal structure, we provide corresponding cryoTEM images of a representative liposome at low and high magnification.

FAQ

Can Vironova Bioanalytics provide analysis on filled and empty liposomes?

We can provide filled and empty analysis of liposomes using cryo-TEM, which allows us to visualize the encapsulant, such as Doxorubicin, inside the liposome. In addition to morphological observations, our analytical report also includes statistical results, such as the ratio of filled and empty liposomes. 

Can Vironova Bioanalytics perform the stability study of liposomes?

We can perform stability studies of liposomes. Contact us to learn more about our stability study services for liposomes and to discuss your requirements.

What is the volume and particle concentration of the liposomal product required to perform cryo-TEM analyses?

We require a minimum sample volume of 25 µL and 2 mL of buffer for cryo-TEM analysis, with a recommended lipid concentration of ca. 4 mg/mL for liposome samples.

At which temperatures can Vironova Bioanalytics store liposome samples?

We offer sample storage at four different temperatures: -86°C, -20°C, +4°C, and room temperature.

Need Quality Control with TEM Analysis  

Analyses for Liposomes

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