Figure 1. Morphology of empty liposome and liposome with drug crystal inside.
Figure 2. The histogram representing packaging of liposomes obtained from semi-automated detection, see detected image with the detected particles overlayered with blue/green/red circles).
Figure 3. Morphology of liposomes portraying different lamellarities.
Figure 4. The histogram representing lamellarities obtained from semi-automated detection, see detected image with the detected particles overlayered with red/yellow/green circles).
Figure 5. The histograms representing size distribution (left) and internal volume (right) obtained from semi-automated detection (about 1500 particles were detected for the analysis).
Figure 6. CryoTEM image (right) of liposomes containing Doxorubicin and their circularity histogram (left).
Figure 7. Histogram representing the distribution of liposomal bilayer thickness (left) and cryoTEM images of liposomes at low magnification (middle) and high magnification (right).
Please see a detailed and more profound description of the different analyses we offer in our electron microscopy services below.
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Yes, Vironova EM services provides full and empty analysis of liposomes by using cryoTEM. CryoTEM allows visualization of the encapsulant (e.g., Doxorubicin) inside the liposome. In the analytical report, we provide the morphological observations, as well as the statistical results (e.g., ratio of full and empty liposomes).
Yes, Vironova can perform the stability study of liposomes. The preferred types of analysis can be discussed with our senior scientists at EM services, please contact us for more information.
We require a minimum volume of 50 µL of sample and 2 mL of buffer, in case dilutions are needed. The sample concentration is dependent on different factors, particularly sample composition. Liposomes samples require a lipid concentration of ca. 4 mg/mL for cryoTEM analysis.
At our facilities, we can store your samples at 4 different temperatures: ―75 °C, ―20 °C, +4 °C, and room temperature.
Contact us if you have any questions about the content on this page or anything else related to our electron microscopy services.
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