Step 1
Acquire grid-square images at large field-of-view. The system automatically adjusts to grid orientation.
A powerful, semi-automated and versatile system for analysis and characterization of nanoparticles, viral-based biotherapeutics and other applications in biotherapeutic development and production.
An in-house solution for TEM analysis. for direct analysis of product quality during manufacturing.
Intuitive systems with automation with powerful AI analysis for non-expert users.
Rapid method suitable for routine daily analysis. Only small volume of product needed.
One method answers many questions about the product. Reducing risk, saving time, and money.
Offers an in-house solution for TEM analysis. for direct analysis of product quality during manufacturing.
Intuitive systems with automation for non-expert users with powerful AI analysis.
ViroTEM offers a powerful and in-house analytical solution enabling electron microscopy experts and non-experts in the biopharmaceutical industry to rapidly obtain meaningful nanoparticle characterization data and insights.
The system is powered by our software VIAS, which automatically images, detects, and analyzes particles with defined morphological characteristics. It further transforms the data into accurate metrics which will help you to get meaningful insights.
The ViroTEM analysis system comprises:
1. A 120 kV transmission electron microscope (TEM) with multiple lens configurations, including a standard lens for unsurpassed high contrast and a class-leading HR lens for high resolution. This innovative design allows for a wide variety of specialized applications.
2. Vironova Imaging and Analysis Software (VIAS) which provides semi-automatic image acquisition as well as subsequent analysis and data presentation. Pattern recognition and machine learning capabilities enable VIAS to perform advanced particle characterization, classification, and measurements.
The combination of the advanced TEM system together with VIAS software enables automated, 2-level screening which allows the system to determine which grid squares are suitable for imaging and then acquire a series of high-resolution images at a pre-determined number of locations on the grid.
Figure 1 - The ViroTEM system is a versatile TEM-based analysis systen designed for simple operation but with powerful functionality.
The semi-automated, multilevel screening functionality of the ViroTEM involves four main steps:
Acquire grid-square images at large field-of-view. The system automatically adjusts to grid orientation.
The system compares each image to reference image(s) to assess suitability for imaging.
High-resolution images are obtained from grid squares that have been given a high suitability rating.
Images are analyzed to identify (i.e. segment) and classify particles.
The ViroTEM system translates TEM image data into quantitative analytics which can be used to make informed decisions in process development and manufacturing. Figure 3 shows an example of an analysis performed on adeno-associated viruses (AAVs).
Figure 3 - Analysis of internally-stained versus non-stained AAVs. Internally-stained vectors can be correlated to a loss of vector-integrity.
The ViroTEM analysis systems stores data in a secure SQL database structure. The database can be located locally on the control PC, or can be installed in a network environment.
To make good decisions in the manufacturing of gene therapy and vaccine vectors, scientists need high-quality analytical information, immediately. Transmission electron microscopy (TEM) enables direct visualization of viral vectors and their assessment by a comprehensive morphological characterization. Using our intuitive ViroTEM system for the analysis of viral vectors allows for in-house monitoring of product contamination, purity and morphology by non-experts rapidly, saving time and money, and helping deliver a safe and well-characterized final product.
The ViroTEM analysis system is a powerful 120 kW tool for biotherapeutic application methods, especially those involving viral structures, including:
Contact
Vironova AB
Gävlegatan 22
113 30 Stockholm
SWEDEN
E-mail: info@vironova.com