MiniTEM is the innovation that puts the power of TEM technology in your hands
- Rapid nanoparticle characterization
- Meaningful data on particle morphology, size distribution and purity
- Easy to use and place
Developed specifically for nanoparticle characterization
- Resolution of 1 nm
- Accelerating voltage 25kV
The system can be plugged into a standard wall socket and can be placed in any standard laboratory setting – close to your process.
Images and quantitative data on nanoparticles
- Overall morphology and particle integrity
- Particle size distribution
- Particle clustering or aggregation
Automated imaging and image analysis
The automation aspect of MiniTEM provides automatic image acquisition and subsequent analysis and data presentation. Pattern recognition and machine learning capabilities enable MiniTEM to perform advanced particle characterization, classification and measurements. The results are statistically relevant thanks to the automatic acquisition and analysis of a large number of images.
(A) Waypoints are selected. (B) Images are automatically acquired in selected waypoints. (C) A large number of images are obtained. (D) Particles are detected and classified in the images. (E) Defined morphological characteristics are transformed into accurate metrics.
MiniTEM in process development
MiniTEM complements existing routine analysis methods in process development. The system offers an in-house solution that enables non-experts in electron microscopy to rapidly obtain meaningful nanoparticle characterization data. It automatically images, detects and analyzes particles. In a few hours you generate quantitative data and images of your nanoparticles that can support decision making in the optimization of your process.
- Instant results – adjust your process within a few hours
- No waiting for service supplier
- Automation frees up your time for other tasks
- See your product and your process
- Objective quantitative data
- Confirm critical quality attributes
Makes it easier
- Less focus on the technology and more on the results
- Automation allows non-expert operation
- Minimized risk of human error
Gene therapy & Viral based Vaccines
MiniTEM automates imaging, AAV particle detection and measurements to present accurate size distribution data
MiniTEM™ can reveal contaminants in AAV samples, for example proteosomes in the figure below.
In the above graph an overlay of the results from MiniTEM and DLS analysis of the same sample is shown. DLS was in this case not able to distinguish between AAV particles and the proteasomes.
Size distribution study with MiniTEM can also reveal small size debris and aggregates
The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to what is revealed in the acquired images.
Automated size distribution analysis can be used to detect level of debris and compare the purity of different AAV samples
The box-plot shows purity measurement of the two samples calculated as the ratio of the total area of debris to Adenovirus particles.
MiniTEM can be used to automatically quantify disrupted empty AAV particles
TEM analysis of negatively stained AAV samples (nsTEM) is a reproducible method for revealing particles with disrupted capsid structure. Disrupted particles take in stain and can therefore be identified by their darker centres.
Comparing particle integrity of two AAV samples in MiniTEM:
Comparing particle integrity of Adenovirus samples in MiniTEM:
Below is an analysis of MuLV (murine leukemia virus). The numbers of circular intact particles with preserved morphology, of non-circular particles morphologically affected by the processing, and of debris, are compared.
Sample: Myoviridae Coliphage - Isolated by the Brouns lab, Kavli Institute - BN/TU Delft
MiniTEM is employed for size distribution analysis of recombinant proteins to detect deviations in size
The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to the content of the acquired images.
TEM imaging of different samples of monoclonal antibodies (mAbs)
Single mAbs in a monolayer can be observed as well as stress induced aggregates.
Retrovirus detection in cell sections with MiniTEM
MiniTEM can be set to automatically search for retroviruses in cell section by using machine learning technology and template matching
You can use MiniTEM for tissue section analysis at different levels of automation
|Renal pathology||Cilia dysfunctionality|
Automated MiniTEM size analysis of nanoparticles in material science is 5-10 times faster than manual TEM.
A comparative study of MiniTEM versus DLS and NTA
A comparative study of MiniTEM versus DLS Zetasizer Nano and NTA NanoSight NS300 performance when analyzing < 50 nm particles.
MiniTEM speeds up ‘time-to-insight’:
A metric for sample purity based on the ratio of the total area of debris to that of intact Adenovirus particles is presented in this study.
Automated integrity analysis
Automated integrity analysis of AAV and Adenovirus particles using MiniTEM