Automated transmission electron microscopy analysis

MiniTEM is the innovation that puts the power of TEM technology in your hands

  • Rapid nanoparticle characterization
  • Meaningful data on particle morphology, size distribution and purity
  • Easy to use and place

MiniTEM is the innovation that puts the power of TEM technology in your hands.

Developed specifically for nanoparticle characterization

  • Resolution of 1 nm
  • Accelerating voltage 25kV

The system can be plugged into a standard wall socket and can be placed in any standard laboratory setting – close to your process.

Images and quantitative data on nanoparticles

  • Overall morphology and particle integrity
  • Particle size distribution
  • Purity
  • Particle clustering or aggregation

Automated imaging and image analysis

The automation aspect of MiniTEM provides automatic image acquisition and subsequent analysis and data presentation. Pattern recognition and machine learning capabilities enable MiniTEM to perform advanced particle characterization, classification and measurements. The results are statistically relevant thanks to the automatic acquisition and analysis of a large number of images.

Automated imaging and image analysis

(A) Waypoints are selected. (B) Images are automatically acquired in selected waypoints. (C) A large number of images are obtained. (D) Particles are detected and classified in the images. (E) Defined morphological characteristics are transformed into accurate metrics.

MiniTEM in process development

MiniTEM complements existing routine analysis methods in process development. The system offers an in-house solution that enables non-experts in electron microscopy to rapidly obtain meaningful nanoparticle characterization data. It automatically images, detects and analyzes particles. In a few hours you generate quantitative data and images of your nanoparticles that can support decision making in the optimization of your process.

Saves time

  • Instant results – adjust your process within a few hours
  • No waiting for service supplier
  • Automation frees up your time for other tasks

Increases reliability

  • See your product and your process
  • Objective quantitative data
  • Confirm critical quality attributes

Makes it easier

  • Less focus on the technology and more on the results
  • Automation allows non-expert operation
  • Minimized risk of human error

Gene therapy & Viral based Vaccines

Size distribution analysis
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MiniTEM automates imaging, AAV particle detection and measurements to present accurate size distribution data

MiniTEM™ can reveal contaminants in AAV samples, for example proteosomes in the figure below.


In the above graph an overlay of the results from MiniTEM and DLS analysis of the same sample is shown. DLS was in this case not able to distinguish between AAV particles and the proteasomes.

Size distribution study with MiniTEM can also reveal small size debris and aggregates

Level and size of aggregates

The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to what is revealed in the acquired images.

Purity analysis
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Automated size distribution analysis can be used to detect level of debris and compare the purity of different AAV samples

Level of debris

The box-plot shows purity measurement of the two samples calculated as the ratio of the total area of debris to Adenovirus particles.

Virus integrity status
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MiniTEM can be used to automatically quantify disrupted empty AAV particles

Virus particle morphology

TEM analysis of negatively stained AAV samples (nsTEM) is a reproducible method for revealing particles with disrupted capsid structure. Disrupted particles take in stain and can therefore be identified by their darker centres. 


Comparing particle integrity of two AAV samples in MiniTEM:

Integrity graphs

Integrity states


Comparing particle integrity of Adenovirus samples in MiniTEM:

Integrity ratio


Below is an analysis of MuLV (murine leukemia virus). The numbers of circular intact particles with preserved morphology, of non-circular particles morphologically affected by the processing, and of debris, are compared.

MuLV debris

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Sample: Myoviridae Coliphage - Isolated by the Brouns lab, Kavli Institute - BN/TU Delft


Particle size distribution of recombinant proteins
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MiniTEM is employed for size distribution analysis of recombinant proteins to detect deviations in size

The size distribution graph shows the number of particles of expected size as well as smaller size debris and aggregates – the data is easily correlated to the content of the acquired images.

Aggregate & particle detection
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TEM imaging of different samples of monoclonal antibodies (mAbs)

Single mAbs in a monolayer can be observed as well as stress induced aggregates.

Virus detection in tissue sample
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Retrovirus detection in cell sections with MiniTEM

MiniTEM can be set to automatically search for retroviruses in cell section by using machine learning technology and template matching


Applications in pathology
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You can use MiniTEM for tissue section analysis at different levels of automation

 Renal pathology  Cilia dysfunctionality
 Muscular diseases  Neuropathology

Material science

Size distribution
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Automated MiniTEM size analysis of nanoparticles in material science is 5-10 times faster than manual TEM.


Gold nanoparticles

Calcium carbonate

Particle size A
Particle size B

Learning center


Automated purity and integrity check of gene therapy vectors

Unmatched viral particle analysis in vaccine and gene therapy development

Meaningful insights on subvisible particle morphology, size and purity

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